Quantitative determination of ophiopogonin D and ophiopogonin D′ in Fibre Ophiopogon enriched extract by macroporous resin / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-576009
ABSTRACT
AIM:
To establish a HPLC-ELSD method for determining ophiopogonin D and ophiopogonin D′ in Fibre Ophiopogon enriched extract by macroporous resin.METHODS:
The determination was performed by HPLC-ELSD on APOLLO C_(18) column(100 A,5 ?,4.6 mm?250 mm),using acetonitrile-H_2O(50∶50) as a mobile phase,flow rate was at 1.0 mL/min,column temperature was at 25℃,detectorELSD(Neu70℃,Eva90℃,gas flow1.5 SLM).RESULTS:
The linear range of ophiopogonin D was 4.424-22.12 ?g,r=0.999 7,The average recovery was 97.6%,RSD=1.51%.The linear range of ophiopogonin D′ was 3.848-19.24 ?g,r=0.999 8.The average recovery was 98.4%,RSD=4.8%.CONCLUSION:
The method is convenient,sensitive and accurate.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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