STUDY ON THE APOPTOSIS OF RENAL CELLS OF CARBON TETRACHLORIDE INDUCED RATS / 解剖学报

ABSTRACT

Objective To observe the change of renal morphological structure and apoptosis of renal cells. Methods Healthy adult male Wistar rats were randomly divided into control group(n=5),2 weeks CCl_4 group(n=5) and 6 weeks CCl_4 group(n=5).The control group were injected oily solution with 0.12ml/100g rat weight,the CCl_4 group were subcutaneously injected 60% carbon tetrachloride oily solution with 0.3ml/100g rat weight.At the first day of 3 weeks and 7 weeks,2 weeks CCl_4 group and 6 weeks CCl_4 group rats were respectively killed.The morphological structure in renal central section was observed after HE staining.The expressions of Caspase-3 and Bax were detected by immunohistochemistry.The apoptosis of rat renal cells was detected by TUNEL staining. Results The structures of rat renal tubules and corpuscle was normal in the control group.We detected the histological change in proximal tubules in the 6 weeks CCl_4 group,tiny tomentums of epithelial cells fell off,cells was vacuolization.The structure of renal corpuscle was normal.The pathological changes of 2 weeks CCl_4 group were similar,but relative little and limited.There was no Caspase-3 and Bax positive cell in control group and 2 weeks CCl_4 group,but lots of Caspase-3 and Bax positive cells were in 6 weeks CCl_4 group,mainly fastened on epithelial cells of proximal tubules near the medulla,and brown granules were mostly located in the cytoplasm.There was no positive cell in control group by TUNEL staining.There were a lot of apoptosis epithelial cells of proximal tubules,renal glomerular also had a few of TUNEL positive cells in the 2 weeks CCl_4 group.Apoptosis cells mainly located in the epithelial cells of distal tubules in the 6 weeks CCl_4 group,a few of apoptosis epithelial cells were in proximal tubules.Conclusion In carbon tetrachloride induced renal injuried rats,apoptosis cells mainly happened in epithelial cells of proximal tubules in 2 weeks.The apoptosis of epithelial cells of distal tubules presented 6 weeks later.