CONSTRUCTION,EXPRESSION AND DETECTION OF THE EUKARYOTIC EXPRESSION VECTOR OF SEROTONIN N-ACETYLTRANSFERASE GENE / 解剖学报
Acta Anatomica Sinica
; (6)2002.
Article
in Zh
| WPRIM
| ID: wpr-576049
Responsible library:
WPRO
ABSTRACT
Objective To investigate the possibility that the construction and expression of a eukaryotic expression vector system of rat NN-NAT gene. Methods The full-length cDNA fragment of rat AA-NAT gene was amplified by RT-PCR method.After retrieving the PCR products,ligating it with pTARGET~(TM) vector,transformating ligation reaction to JM109 huge efficiency competent cells and identifying the recombinant plasmid,the recombinant eukaryotic expression vector pTARGET~(TM)-AANAT was transfected into rat L6 myoblasts with lipofectamine.Accordingly,engineered cells selected by antibiotic G418 were detected by the methods of RT-PCR and Westem blotting. Results It was revealed that,amplified AA-NAT cDNA confirmed by agarose gel electrophoresis could ligate with pTARGET~(TM) vector and subcloned into JM109 cells.L6 cells transfected with pTARGET~(TM)-AA-NAT survived well after G418 selection and expressed AA-NAT protein. Conclusion Our results suggest that we have prepared rat AA-NAT stable eukaryotic expression system successfully although it was just a primary result.This system can be used for the transfection of L6 myoblast.
Full text:
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Index:
WPRIM
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Acta Anatomica Sinica
Year:
2002
Type:
Article