Determination of ginsenoside Rg1 and notoginsenoside R1 in Yiqi Gushen Dialysis Liquid by solid phase extraction and TLC densitometric method / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-576133
ABSTRACT
AIM:
To establish the method of determining ginsenoside Rg1 and notoginsenoside R1,and control the quality of Yiqi Gushen Dialysis Liquid(Radix et Rhizoma Notoginseng,Radix Astragali,etc.)METHODS:
Sample was pretreated with solid phase extraction using ODS C_13 cartridges.Ginsenoside Rg1 and Notoginsenoside R1 were separated with TLC method.The mobile phase was chloroform-methanol-water(3∶0.8∶0.1),developed twice.The coloration was 10% sulphuric acid ethanol solution.The detection wavelength was 560 nm.RESULTS:
The calibration curves for ginsenoside Rg1 and notoginsenoside R1 were A=113.4 C-34.976,(r=0.997),A=92.14 C-13.597,(r=0.997),respectively,the linear ranges were between 2.64-13.2 mg and 0.85-8.5 mg,and the detection limit was 0.88 mg and 0.65 mg,respectively.The average recoveries were 100.31% and 98.72%,respectively.The contents of the sample were between 5.683-5.764mg,1.528-1.599 mg per 10 mL,respectively.CONCLUSION:
The proposed method for estimating ginsenoside Rg1 and notoginsenoside R1 is simple,precise,specific,sensitive and accurate and can be used for quality control of Yiqi Gushen Dialysis Liquid.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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