The effect of NOS inhibitors on fetal rat neuron apoptosis during intrauterine distress and childhood's learning and memory / 重庆医科大学学报

ABSTRACT

Objective:Evaluating the effect of nitric oxide synthase(NOS) inhibitors on fetal rat neuron apoptosis during intrauterine distress and childhood's learning and memory.Methods:① Set up fetal rat intrauterine distress model.②Divide into the control group,reperfusion group,treatment group [injected N omega-nitro-L-arginine(L-NNA) 4mg/kg into pregnant rat's abdomen before ischemia and injected aminoguanidine(AG) 500mg/kg before operation],long-term observation group Ⅰ?Ⅱ?Ⅲ(the part of rats in control group,reperfusion group,treatment group were performed cesarean section on pregnant 19 days and bred to 40 day of age after delivery).③Measuring the content of cytochrome c in cytosol and examined the ability of learning and memory function.Results:①The content of cytochrome c in reperfusion group(6/12/24h) and treatment group(6/12/24h) were higher than those in the control group(P0.05).(3) Crossing platform times in long-term observation groupⅡ were fewer than those in long-term observation group Ⅰ?Ⅲ(P 0.05).Conclusion:NOS inhibitors play a obvious protective role in long-term intelligence of fetal rat after delivery through relieved neuron apoptosis during hypoxic ischemia encephalopathy.NOS inhibitors can be a valuable therapy method in intrauterine distress treatment.