The effects of IL-1 beta on the differentiation of rats NSCs into dopaminergic neurons in vitro / 重庆医科大学学报

ABSTRACT

Objective:To explore the effects of IL-1 beta on the differentiation of NSCs into dopaminergic neurons.Methods: To isolate and culture mesencephalic neural stem cells in vitro.we used immunocytochemistry to detect TH-positive neurons induced by 10%FBS and interleukin-1 bate for 7days respectively.Results: The positive ratio of TH expression in induced cells by 10%FBS,IL-1? 10pg/ml,IL-1? 120pg/ml,IL-1? 150pg/ml,IL-1? 200pg/ml were 0.45%,0.6%,7.2%,12.5%and 8.75% respectively.The positive ratios of TH expression in induced cells by IL-1? 150pg/ml(12.5%) were much higher than that by 10%FBS(control 0.45%)(P