Construction of human single-chain antibody gene for breast cancer / 重庆医科大学学报

ABSTRACT

Objiective:To construct human single-chain antibody gene fragment for breast cancer.Methods:Total RNA was isolated from tumor adjacent lymphatic tissue of breast cancer patients.Then heavy and light chain(VH and VL) genes of antibody were amplified separately by RT-PCR,and assembled into ScFv genes by SOE-PCR.Results:The human single-chain antibody gene fragment for breast cancer was constructed and its size was about 750bp.Conclusion:The human single-chain antibody gene fragments were constructed successfully,which provides a way for constructing the human single-chain antibody library of anti-breast cancer.