Chromatographic studies on fingerprints of Cortex Magnoliae officinalis and their processed products by HPLC-DAD-MS / 中成药

ABSTRACT

AIM: To establish chromatogram figerprints of fresh Magnoliae officinalis and their processed product by HPLC-DAD-MS method. METHODS: A HPLC-DAD-MS method was proposed for quality assessment of Magnoliae officinalis and their processed product,the HPLC columm,mobile phase,elution mode and gradient program were optimized in order to obtain high quality HPLC profile.HPLC analysis was performed on a LC-18 colunm(5 ?m,250 mm?4.6 mm) with the mixture of H_2O(A) and acetonitrile(B) as mobile phase in gradient mode.The colunm temperature was set up at 30 ℃ and the flow rate was 1 mL/min. RESULTS: The HPLC fingerprint was established based on the analysis of ten fresh Magnoliae officqinalis herbal samples.Twenty-two characteristic peaks were found in all ten samples.The main characteristic peaks were identified based on the comparison of UV and MS spectra of each analyte with that of authentic compounds and literature data.The HPLC fingerprint of processed Magnoliae officinalis was established.Comparing with corresponding fresh herbal samples,twent-three characteristic peaks were found in all ten processed herbal samples.Moreover,gingerol was found in processed Magnoliae officinalis. CONCLUSION: The fingerprint chromatograms from HPLC method can be used to evaluate the quality of Magnoliae officinalis and their processed products.