Analysis of the volatile compounds in Liushen Pill and Japannese Jiuxin Pill by GC/MS / 中成药

ABSTRACT

AIM: To develop a GC/MS method for analyzing volatile compounds in Liushen Pill and Japanese(Jiuxin) Pill. METHODS: The gas chromatograph was fitted a fused-silica capillary column with a VF-5 ms stationary phase and dimensions:30 m?0.25 mm i.d.,0.25 ?m film thickness.The carrier gas was helium at a flow-rate of 1 mL/min.Injector temperature was at 280 ℃ and operated in the splitless mode.The temperature of the GC-MS transfer line was at 250 ℃.Full scan electron impact ionization data were acquired under the following condition:solvent delay 6 min.70 eV electron impact energy,scan time 0.87 sencond/scan,emission current 80 mA,manifold and trap temperatures 45 ℃ and 170 ℃. RESULTS: The quantitative method was accepted with good specificity,wide linear range and low LOD.At the same time,the content of isoborneol was determined by a semi-quantitative approach. CONCLUSION: The research is propitious to offer an effective and reliable pattern for analyzing volatile compounds in complex composite formulae.