Establishment of allergic airway response model in mice with intestinal microflora imbalance induced by dermatophagoides pteronyssinus allergens / 重庆医科大学学报

ABSTRACT

Objective:To establish the intestinal microflora imbalance mouse model to construct a novel allergic airway response model induced by dermatophagoides pteronyssinus allergens. Methods:Sixty BALB/c mice were divided into six groups. Mice in two groups were prepared for the analysis of the quantity of the intestinal bacterial flora and mice in other four groups for the analysis of allergic airway response. Results:Intestinal microflora of mice in antibiotic therapy group presented the weight loss and feces moisture increase. For the mice with intestinal microflora imbalance and induced by dermatophagoides pteronyssinus allergens,the number of total cells,lymphocytes,neutrophils and eosinophils in bronchoalveolar lavage fluid(BALF)significantly increased,the expression level of IL-4 increased,and the expression of GATA-3 mRNA in the lung tissue were upregulated. Conclusions:An allergic airway response model was established successfully by using dermatophagoides pteronyssinus allergens to induce mice with intestinal microflora disruption.