EFFECTS OF RNAi-MEDIATED GENE SILENCING OF E-CADHERIN EXPRESSION ON THE BIOLOGIC BEHAVIORS OF HO-8910 CELLS / 解剖学报
Acta Anatomica Sinica
;
(6)1954.
Article
in Chinese
| WPRIM
| ID: wpr-576529
ABSTRACT
Objective In many types of epithelial tumors,down-regulation or mutation of the epithelial cell-adherent molecule E-cadherin is associated with an increased invasiveness that can be prevented by the forced expression of the cell-adherent molecule.This suggests that E-cadherin is a latestage tumor suppressor that prevents invasion and metastasis.This study was to investigate cell invasion and migration status of human ovary serous cystadeno carcinoma HO-8910 cell line when the E-cadherin expression was down-regulated with RNA interference(RNAi) technology. Methods E-cadherin siRNA was transfected into HO-8910 cells to inhibit the expression of E-cadherin.The effect of RNAi was detected by immunofluoresence assay and Western blotting.The invasive ability of the cancer cells was determined by Transwell assay. Results After RNAi,the expressions of E-cadherin were significantly decreased from 63.7% to 11.9%(P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Anatomica Sinica
Year:
1954
Type:
Article
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