Method Research of Measuring Soluble Protein Contents of Animal Material Rough Extraction Using Coomassie Brilliant Blue / 中国中医药信息杂志
Chinese Journal of Information on Traditional Chinese Medicine
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-576566
ABSTRACT
Objective To study the stability of the Bradford method for determination of soluble protein in animal materials. Method The absorption of CBB G-250 combined with proteins was determined by the UV-Spectrophotometry method with the detection wavelength at 595 nm,and the dried bufo was selected as sample,BSA as control. Results The method showed a good linear relationship between absorption and protein content (r =0.996). The sample was stable with in 10 min (RSD=5.0%). Conclusion This method was reliable,simple and rapid.
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Information on Traditional Chinese Medicine
Year:
2006
Type:
Article
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