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Determination of AstragalosideⅣin Ejiao Buxue Koufuye by HPLC-ELSD / 中国中医药信息杂志
Article in Zh | WPRIM | ID: wpr-576664
Responsible library: WPRO
ABSTRACT
Objective To establish a method for the determination of AstragalosideⅣin Ejiao Buxue Koufuye.Methods HPLC-ELSD was used to determine directly AstragalosideⅣin Ejiao Buxue Koufuye on Betasil C_(18) column,using CH_3OH-H_2O(74:26)as a mobile phase.The flow rate was 1.0mL/min.The temperature of drift tube for ELSD was 90℃and the flow rate of N_2 was 2.0L/min.Results The linear range of AstragalosideⅣwas 0.43~2.15?g,Y=1.53637X+0.92158,r=0.9999(n=6),the average recovery of the added sample was 98.5% and RSD=0.98%.Conclusion The method is accurate and reliable with a good reproducibility.It can be used for the quality control of Ejiao Buxue Koufuye.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Information on Traditional Chinese Medicine Year: 2006 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Information on Traditional Chinese Medicine Year: 2006 Type: Article