Analysis of Epstein-Barr virus LMP1 gene Xhol-site mutation in plasma of patients with nasopharyngeal carcinoma in Chongqing of China / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-576671
ABSTRACT
Objective:
To detect and analyze the Epstein-Barr virus LMPI gene XhoI-site mutation in plasma of patients with nasopharyngeal carcinoma(NPC)in Chongqing of China.Methods:
DNA extraction and PCR amplification was used in plasma of 48 NPC patients and 40 control non-NPC cases from Chongqing of China.All the PCR production was digested by enzyme XhoI,then segregated in 8% PAGE.XhoI-site mutation was analyzed by sequencing and comparing with B95-8 cell.Results:
The LMP1 was amplified and digested successfully from 48 of 48 NPC cases(100%)and from 38 of 40 non-NPC cases (95%),but none of LMPI XhoI-site mutation was found.Sequencing was performed on 4 LMPI genes from NPC cases and 5 LMP1 genes from non-NPC cases,they showed the presence of the XhoI-site.Conclusions:
XhoI-site in EBV LMP1 gene N terminus has no mutation in plasma of NPC or non-NPC cases in Chongqing of China.The precise relationship of XhoI-site mutation and NPC pathogenesis need further investigation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2003
Type:
Article
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