In-vitro Inhibitory Effect of Phyllanthus Urinaria L Compound on Proliferation of Human liver Cancer Cell HePG_2 and Its Apoptosis Induction / 中药新药与临床药理

ABSTRACT

Objective To investgate the inhibitory effect of Phyllanthus Urinaria L compound on proliferation of hepatoma cell HePG2 in vitro and to explore its mechanism.Methods The influence of different concentrations of Phyllanthus Urinaria L compound at different time on HePG2 proliferation was compared by MTT colorimetric assay and cell growth curve assay.The cell apoptotic rate and morphological changes of HePG2 were observed by flow cytometry,fluorescence microscope and electron microscope.Results Phyllanthus Urinaria L compound can inhibit the proliferation of human hepatoma cell line HePG2.Within a certain limit of concentrations,the higher the concentration and the longer the acting time,the stronger the inhibition.Co-cultured with 500 ? g/mL Phyllanthus Urinaria L compound for 72 h,the inhibitory rate reached 93.58 % and IC50 was 240 ? g/mL.Phyllanthus Urinaria L compound at different concentrations had an certain effect on inducing cell apoptosis.Conclusion Phyllanthus Urinaria L compound can inhibit the proliferation of hepatoma cell,and its mechanism may be related with the induction of hepatoma cell HePG2 apoptosis.