The study of correlation between CD40-CD40L system and atherosclerotic plaque stability / 重庆医科大学学报

ABSTRACT

Objective:To study the expression of CD40-CD40L system on rabbit aorta plaque and correlation with plaque stabil- ity during atherosclerotic process.Methods:50 healthy rabbits were derided into 10 groups in randomfive controlled groups (C1～5 group)and five AS model groups(AS1-5 group).Rabbits in C1～5 group were fed on regular diets until 8 weeks,10 weeks.12weeks,14weeks and 16weeks to get the aorta specimen respectly.Correspondingly rabbits in AS1～5 groups were given high lipid diets continously until 8weeks,10weeks,12weeks,14weeks and 16weeks,during which each AS group was conducted balloon injury at the end of the first week to prumote the establishment of AS models.All the groups were detected serum CRP individually at beginning and before specimen obtained.Immunohistochemistry and special stain was used to detect the intima expression proportion of CD40,CD40L,MMP-1 as well as contant of collagen and lipid photographed under microscope and measured by computer.Then the collagen/lipid ratio were calculated.Finally correlation between CD40-CD40L expression and plaque stability were analyzed.Results:All atherosclerotic plaques were successfully established on all rabbits'abdominal aorta in the 5 AS groups.Compared with C1～5 groups correspondantly,the expression of CD40,CD40L,MMP-1 were significantly in- creased(P