Determination of pterodontic acid in Lingdancao Soft Capsula by HPLC / 中草药

ABSTRACT

Objective To establish an HPLC method for determination of pterodontic acid in Lingdancao Soft Capsula. Methods Agilent Zorbax XDB-C18 column (250 mm?4.6 mm, 5 ?m) was used. Acetonitrile-water (60∶40) was used as mobile phase, the detection wavelength was 210 nm, flow rate was 1.0 mL/min, and column temperature was at 25 ℃. Results The linear range of pterodontic acid was 12.91—129.1 ?g/mL (r=0.999 9), the average recovery rate of pterodontic acid was 99.7 %, RSD was 2.0%. Conclusion The method is simple, accurate, and reliable, and it can be used to the quality control of this preparation effectively as a quantitative method.