Effect of VEGF-C ASODN on adhesion and invasion of ovary cancer cells / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effect and the mechanism of VEGF-C antisense oligodeoxynucleotide(ASODN) on the adhesion and invasion of human ovary cancer cell line SKOV3 in vitro.Methods:The ASODN sequence targeting the coding region of VEGF-C was synthesized and introduced into ovary cancer cells by liposomes.The expression of VEGF-C protein was detected by inmmnohistochemistry;the adhesion of SKOV3 cells to artifical basement membrane Matrigel was measured by MTT assay.The invasion was evaluated by transwell chambers attached with polycarbonate filters and reconstituted basement membrane(Matrigel).The expression of MMP-2 protein was measured by western blot.Result:In ASODN group,the adhesive rate and the invasive cell numbers were decreased markedly(P