HPLC fingerprint of amino acids compounds of Xiasangju Granules / 中成药

ABSTRACT

AIM:To establish the fingerprint chromatogram of amino acids compounds of Xiasangju Granules(Spica Prunellae,Flos Chrysanthemi indici,Folium mori.).METHODS:To apply 6-aminoqtiinoly-N-hydroxysuccinimdyl carbamate(AQC) pre-column derivatization HPLC method.Separation was performed on SYMMETRY C_(18)(150 mm?(4.6) mm,5 ?m) analytical column with mobile phase consisting of acetate(pH=(5.05)) and 60% acetonitrile with gradient elution with the flow rate(1.0) mL/min and the column temperature at 37 ℃.The Fluorescence wavelength used for detection was set at 250 nm(Excitation wavelength) and 395 nm(Emission wavelength) and the analysis time was 50 min.RESULTS:12 co-peaks on the HPLC fingerprints of Xiasangju Granules were indicated.The similarities were determined by the coefficients of cosine and correlation.The results of similarity analysis were(0.95)-(1.00).CONCLUSION:Perfect fingerprints were obtained which can be used for the quality control of Xiasangju Granules.