Effect of liquid nitrogen rapid freezing technology on components of water-soluble proteins in ground dragon and their activities / 中草药

ABSTRACT

Objective To study the effect of cold-processing technology on the composition of water-soluble proteins in ground dragon and their activities of thrombolysis and anti-tumor activity.Methods Ground dragon was respectively processed by traditional processing technology and cold-processing technology.The latter was done by the liquid nitrogen rapid freezing technology and lyophilizing technology.By means of qualitative and quantitative tests,SDS-PAGE and the software of ImageTool were applied to analyzing the difference of protein components in ground dragon from the two processing techniques.Fibrin plate method and thrombolysis test in vitro were carried out to determine the difference of the two processing techniques for the fibrinolytic activity.Micro-observation and MTT methods were used to assess the differences of antihepatic cancer activity between the traditional and the cold-processing groups.Results Compared with the traditional group,the components of water-soluble proteins in the ground dragon processed by cold-processing technique were more abundant than traditional one,and the number of protein bands and concentration in SDS-PAGE gel were more and much higher.Although the two kinds of protein solution from the different processing techniques had the visible fibrinolytic activity and antihepatic cancer activity,both activities of cold-processing technique were markedly higher than those in the traditional group.Conclusion Effects of different processing techniques of ground dragon on fibrinolytic activity and anti-tumor activity are significant.On protecting the bioactivity of proteins in ground dragon,the cold-processing techniques is advantage to the traditional processing technology.