FUNCTION OF p21-ACTIVATED KINASE 2 IN XENOPUS OOCYTE CYTOKINESIS / 解剖学报

ABSTRACT

Objective Study the function of p21-activated kinase 2 in Xenopus oocyte cytokinesis. Methods Xenopus oocyte was used as a model,and MPF activity and PAK2 phosphorylation status were observed during oocyte maturation.The specific inhibitor of PAK2 activity,PAK2-NT(PAK2-N-terminal) was used to microinject into Xenopus oocyte.Under fluorescent microscope germinal vesicle breakdown was observed during cytokinesis.To further observe the changes of F-actin and spindle,laser scanning confocal microscopy with time-lapse method was employed. Results Occurrences of germinal vesicle breakdown in control oocytes was similar to those oocytes injected with PAK2-NT mRNA,but no cytokinesis was observed in oocytes injected with PAK2-NT mRNA.Conclusion These results indicated that PAK2 might involved in Xenopus oocytes cytokinesis.