Determination of Syringin and Syringaresinol in Ciwujia Injection by RP-HPLC / 中国中医药信息杂志
Chinese Journal of Information on Traditional Chinese Medicine
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-577000
ABSTRACT
Objective To establish the determination method of syringin and syringaresinol in Ciwujia Injection with RP-HPLC. Methods C18 ODS was used as a stationary phase. The mobile phase consists of water and acetonitrile. The gradient condition was 0→20 min, A90%→50%. The flow rate was 1.0 mL/min. The detective wavelength was set at 220 nm. Results The linear range of syringin was 0.03~0.16 ?g (r =0.999 7, n=5) and the linear range of Syringaresinol was 0.02~0.13 ?g (r =0.999 3, n=5). The average recovery of syringin was 99.34% and RSD=0.58%. The average recovery of Syringaresinol was 99.22% and RSD=0.71%. Conclusions The method was simple, convenient and accurate, and can be used for content determination of syringin and syringaresinol in Giwujia injection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Information on Traditional Chinese Medicine
Year:
2006
Type:
Article
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