Effect of inhibiting c-raf-1 on radiosensitivity in human cervical carcinoma cell line HeLa / 重庆医科大学学报

ABSTRACT

Objective:To explore the effect of inhibiting c-raf-1 by antisense technology on radiosensitivity in human cervical carcinoma cell line HeLa.Methods:There were four groups in the studycontrol group,lipofectin group,sense oligodexynucleotides(S-ODN) group,antisense oligodexynucleotides(AS-ODN) group.The expression of c-raf-1 was detected by means of RT-PCR,Cellular response to irradiation was evaluated by the colony forming test,Apoptosis was observed by fluorescent staining.Bcl-2 protein expression was determined by flow cytometry.Results:AS-ODN group could significantly decreased the proliferation rate and increasing the apoptosis rate,significantly downregulating the expression of Bcl-2 of irradiated human cervical carcinoma cells,(vs lipofectin group or S-ODN group,P