Relationship between AQP9 expression and formation of ischemic cerebral edema in the rats / 重庆医科大学学报

ABSTRACT

Objective:To study the role of AQP9 and its mRNA expression in the formation of ischemic cerebral edema in the rats.Methods:MCAO models were established by occluding unilateral middle cerebral artery(MCA) of the rats with the suture method.The morphological changes were observed with HE stain and transmission electron microscope(TEM).Brain water content(BWC) was measured by using wet-dry weighing method.The expression of AQP9 and its mRNA in the edema brain tissue were detected with in situ hybrilization(ISH) and immunohistochemistry(IHC).Results:The space around the neurons and perivascular space became larger.Some neurons showed necrosis phenotype,and the number of the neurogliocyte increased.The expression of AQP9 and its mRNA were up-regulated in subfornical organ(SFO)、choroid plexes、brain cortex、supraoptic nuclei、hippocampus and other regions,and reached its peak at 48~72h after MCAO.The brain water content(BWC) in ischemic cerebral tissue reached its peak at 72h.Conclusions:the expression of AQP9 and its mRNA were up-regulated following the brain edema.The results imply that AQP9 might play an important role in the formation of ischemic cerebral edema.