Determination of Psoralen and Isopsoralen in Different Effective Extracts of Fructus Psoraleae by RP-HPLC / 中药新药与临床药理
Traditional Chinese Drug Research & Clinical Pharmacology
;
(6)2000.
Article
in Chinese
| WPRIM
| ID: wpr-577198
ABSTRACT
Objective To establish a HPLC method for the assay of psoralen and isopsoralen in different effective extracts of Fructus Psoraleae. Methods HPLC was carried out on the column of Kromasil RP-C18. The mobile phase was methanol -water(65 ∶35). The flow rate was 1.0 mL/min and the UV detection wavelength was 245 nm. Results Good linearity of psoralen was showed within the range of 10.5 ng~525 ng(r= 0.999 3)and isopsoralen within the range of 9 ng~450 ng (r= 0. 999 9). The content of psoralen and isopsoralen differed in different extractions of Fructus Psoraleae. Among them,the extract C (extracted by ethyl acetate ) contained the highest contents of psoralen and isopsoralen,while the contents of psoralen and isopsoralen were very low in the extract D (extracted by n-butyl alcohol) and E (supernatant of water extract). Conclusion The method is simple,accurate and reproducible. The anti-asthma effect and the dose-effect relationship of the different effective extracts of Fructus Psoralea need further pharmacodynamics study.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
2000
Type:
Article
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