Effect of Moschus Combined with Borneol on Brain Water Content and Blood-Brain Barrier Permeability in Rat Model of Cerebral Focal Ischemia with Reperfusion / 广州中医药大学学报

ABSTRACT

【Objective】To observe the effect of Moschus combined with Borneol on brain water content and blood-brain barrier(BBB)permeability in rat model of cerebral focal ischemia with reperfusion.【Methods】Thirty SD rats were randomized into 6 groups sham-operation group,model group,Moschus(1 mg?kg-1?d-1)group,Borneol(3 mg?kg-1?d-1)group,Moschus with Borneol group,nimodipine(12 mg?kg-1?d-1)group.The rat model of cerebral focal ischemia with reperfusion was established with method of putting nylon monofilament in the internal carotid artery.The brain water content was evaluated by detecting the wet weight and dry weight of brain.The detection of cerebral extra-vascular Evans blue(EB)content was used to observe the changes of BBB permeability.【Results】After cerebral ischemia for 2 hours and reperfusion for 24 hours,water content and EB content in the model group increased significantly compared with the pseudo-operation group(P