Determination of Vitexin in Cajanus cajan(L.) Millsp. by HPLC / 中药新药与临床药理
Traditional Chinese Drug Research & Clinical Pharmacology
;
(6)2000.
Article
in Chinese
| WPRIM
| ID: wpr-577433
ABSTRACT
Objective To develop a quantitative method to determine vitexin in Cajanus cajan (L.) Millsp. Methods The chromatographic conditions were as follows column C18(250 mm? 4.6 mm, 5 ? m) , mobile phase being MeOH ∶ 1 % HAC (25 ∶ 75) for 20 mins and then being MeOH ∶ 1 % HAC (30 ∶ 70) after 20 mins, flow rate at 1.0 mL/min, and wavelength at 339 nm. Results The good linearity of this method was in the range of 0.104 4~ 0.522 ? g (r=0.999 4), and the average recovery of vitexin was 100.11 % ( RSD =0.63 % ).Conclusion The method is simple and sensitive with good stability, it can be suitable for the quality control of vitexin in Cajanus cajan (L.) Millsp..
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
2000
Type:
Article
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