Effect of Tongbiding Injection on Expression of N-methyl-D-aspartate Receptor 1 mRNA in the Spinal Cord of Chronic Constriction Injury Rats / 中国中医药信息杂志

ABSTRACT

Objective To study the effect of Tongbiding injection on expression of N-methyl-D-aspartate (NMDA)receptor 1 mRNA in the spinal cord of chronic constriction injury(CCI)rats.Methods Thirty male Spragru-Dawley rats were randomly divided into control group(group A),CCI group(group B)and Tongbiding group(group C),ten rat for eah group.The changes in NR1 mRNA expression in the spinal cord was detected by reverse transcription-polymerase chain reaction(RT-PCR)techniques.Results The expression of NR1 mRNA in the spinal cord was minimal in control group,CCI induced significant increase in the expression of NR1 mRNA in the spinal cord and Tongbiding significantly inhibited the increase in NR1 mRNA expression.There was significant difference between CCI and Tongbiding group(P