In vitro study on Th1/Th2 shift modulated by anti-CTLA4 antibody in unexplained recurrent spontaneous abortion / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effects of anti-CTLA4 antibody on Th1/Th2 shift in unexplained recurrent spontaneous abortion. Methods:The levels of IL-2,IFN-?and IL-4 were assessed by ELISA in the supernatant of cultured maternal peripheral blood mnnonuclear cells(PBMC) from 30 patients of Unexplained RSA. Cytokine synthesis was induced by activation with antigens from a chnriocarcinoma cell line of JEG-3,with anti-CTLA4 antibodv or IgG. Results:We detected significantly greater levels of the Th1 cytokines IL-2、IFN-?(P0.05). Conclusion:Anti-CTLA4 antibody can up-regulate Th1-type cytokines and down-regulate Th2-type cytokines, which indicates that in URSA anti-CTLA4 antibody could swith T cell responses to Thl,and is harmful to URSA.