Histocytological Observation of Somatic Embryogenesis in In-Vitro Cultured Dendrobium candidum wall. Ex Lindl / 广州中医药大学学报
Journal of Guangzhou University of Traditional Chinese Medicine
;
(6)2001.
Article
in Chinese
| WPRIM
| ID: wpr-577589
ABSTRACT
Objective To observe the somatic embryogenesis in in-vitro cultured Dendrobium candidum wall.Ex Lindl.(DCWL),and to supply evidence for its rapid propagation and germplasm preservation.Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants,N6 was used as the basic culture with phytohormone added,and fungal extracts as the elicitor.Protocorm-like bodies and callus of DCWL were used for induction culture.Results Somatic embryogenesis in in-vitro cultured DCWL derived from the epithelial cells or inner cells of callus of DCWL.Conclusion A large amount of buds can be obtained by the induction and culture of protocorm-like bodies and callus of DCWL.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Guangzhou University of Traditional Chinese Medicine
Year:
2001
Type:
Article
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