RP-HPLC Determination of Paeoniflorin in Shiquan Dabu Granules / 中药新药与临床药理

ABSTRACT

Objective To establish a content determination method of paeoniflorin in Shiquan Dabu Granules.Methods A RP-HPLC method was adopted.A Hypersil BDS C18(4.6 mm?200 mm,5 ?m)column served as the stationary phase,methanol-water(1545) as mobile phase,and the detection wavelength was 230 nm.Results Paeoniflorin had a good linearity in the range of 0.104～0.519 ?g(r=0.999 2).The recovery was 98.77 %and RSD was 1.77 %.Conclusion The method is simple,accurate,reproducible,and can be used to control the quality of Shiquan Dabu Granules.