Fingerprint analysis of Spica Prunellae by HPLC / 中草药

ABSTRACT

Objective To establish a method of fingerprint analysis on Spica Prunellae by HPLC.Methods The HPLC method was used,chromatographic conditions were Betasil C18 column(250 mm ? 4.6 mm,5 ?m),acetonitrile-0.1% phosphric acid system as mobile phase,0-50 min acetonitrile 10%→25%,50-60 min acetonitrile 25%→32%;flow rate 0.7 mL/min;detective wavelength at 210 nm,column temperature 25 ℃.Results Establishing the fingerprint of Spica Prunellae,16 common peaks were found in the HPLC fingerprint of Spica Prunellae and good similarities with correlation coefficients over 0.95 were found in the fingerprints between the herbs from different sources and standard fingerprint,which could be utilized for the identification of Spica Prunellae.Conclusion The method is accuate,simple,and could be used for the quality control of Spica Prunellae.