Fingerprints of Herba Ephedrae by HPLC / 中成药

ABSTRACT

AIM:To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Herba Ephedrae. METHODS: HPLC method was set up,using CLCphenyl column(5 ?m 4.6 mm?150 mm) with gradient elution,the mobile phase was comprised of acetonitrile and phosphoric acid(0.1%),the UV detection wavelength was at 215 nm,with a flow rate of 1.0 mL/min. RESULTS: There were 17 common peaks in the fingerprints of Herba Ephedrae based on 10 habitats.Sum of peak areas was larger than 90% total area.The similarity was compared and the HPLC fingerprints was set up. CONCLUSION: The quality of Herba Ephedrae can be controlled effectively by HPLC fingerprints.