Metabolism method of dextromethorphan in vitro / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-577910
ABSTRACT
Objective:
To investigate in vitro metabolism method and HPLC method for the dextromethorphan.Methods:
Total protein of normal liver specimens metabolismed dextromethorphan in vitro.An HPLC method with fluorescence was developed to study the contents of dextromethorphan and metabolites by joining an internal standard(Adriamycin).Methanol-10mmol/l,sodium dihydrogen phosphate solution(5050,contain 0.01% sodium heptanesulfonate,5% triethylamine,pH 3.5)was used to the mobile phase.Results:
Total protein of normal liver specimens metabolismed dextromethorphan in vitro efficiently with coenzyme,Mg~(2+), suitable temperature and pH value.Dextromethorphan and metabolites were analyed by HPLC method with fluorescence.The metabolic rate of dextromethorphan was 16.09%.Conclusion:
It is suggested that the metabolism method of dextromethorphan in vitro is simple,convenient and accurate.It can be used for the determination of metabolism dextromethorphan in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
1986
Type:
Article
Similar
MEDLINE
...
LILACS
LIS