Bioinformatics analysis of the transcriptional regulation of Visfatin / 重庆医科大学学报

ABSTRACT

Objective:To map the approximate transcription factors binding site of Visfatin and identify its potential promoter region.Methods:All reported transcripts of Visfatin were collected through searching online database.Visfatin gene were analysed by CpG/CpGReport/Isochore and FirstEF to predict the CpG island and possible first exon,respectively.The Visfatin gDNA sequence was also analysed by softwares such as PromoterScan to predict the probable promoter region.Matlspector、TFSEARCH and Match were used to predict potential transcription factors binding sites.Results:Among all the reported transcripts of Visfatin,5'UTR of NM_005746 Was the longest.Visfatin gene Was a typical CpG island associated gene.Promoters were predicted in the NM_005746 5'upstream 229～798bp region.In this region,a lot of cis-acting element were found. Conclusion:The transcription initiation site of Visfatin have been indentifiod.A lot of transcription factors binding sites were predict in the NM 005746 5' upstream 229-798bp region.It is important to definite those transcription factors binding sites through experimental methods.