Establishment of acute cerebral venous occlusion and evaluation of it with magnetic resonance imaging / 重庆医科大学学报

ABSTRACT

Objective:To establish a stable animal model of acute cerebral venous occlusion and to evaluate the model with DWI,PWI,MRS and DTI in observing the changing regularity of the brain parenchymal lesions.Methods:Thirty cats were randomly divided into 2 groups,operation group(n=24) and sham operation group(n=6).Operation group was performed by injection of the cellulose acetate polymer(CAP) solution into the superior sagittal sinus(SSS) to establish the acute cerebral venous occlusion,while sham operation group underwent only the exposure of SSS.MRI was performed at 1,3,6,12,24 and 48h after operation.Results:Twenty-two cats in operation group were operated successfully.Twenty-five lesions were detected on DWI in 14 cats.Thirty-one abnormal perfusion regions were detected on PWI in 17 cats.MRS showed that Lac increased obviously in 1h after operation and decreased gradually later,and NAA decreased in 3h.DTI showed that ADCav values decreased in 1h,increased 3h later and FA values decreased at all time points after operation.The microscopic changes were mainly vasogenic edema and followed by venous cerebral infarction and cerebral hemorrhage in 12h.No abnormal findings were observed in sham operation group.Conclusion:Establishment of acute cerebral venous occlusion model by injection of CAP solution into SSS is feasible.The model is suitable for pathophysiological and radiological studies.DWI combined PWI,MRS and DTI have great value in judging the dynamic changes and the etiopathogenesis of brain parenchymal lesions in acute cerebral venous occlusion.