Methylation of FANCF gene plays an important role in the development of cervical cancer / 重庆医科大学学报

ABSTRACT

Objective:To evaluate the disruption of FANCF gene by methylation in cervical cancer and analyze the relation between cervical cancer and the methylation of FANCF DNA so as to find a method to inhibit the development of cervical cancer.Methods:The level of FANCF mRNA was detected with reverse transcription polymerase chain reaction(RT-PCR) before and after treatment with 5-aza-2'-deoxycytidine(5-Aza-CdR).Protein was tested by Western Blot.Methylation specific PCR(MSP) was used to check whether it was methylated in exon of FANCF.Normal cervical cancer tissues were used as controls.Results:The expression of FANCF mRNA in cervical cancer cell line SiHa was absent,and so was the expression of protein.The expression of FANCF mRNA and protein was decreased in Hela cell line.After treated with(5-Aza-CdR),SiHa expressed FANCF mRNA and protein,and time for SiHa cells to proliferate double was prolonged.Compared with the controls,the expression of FANCF mRNA in cervical cancer tissues decreased significantly or absolutely defaulted,and expression of FANCF protein also decreased(P