Effects of Puerarin on Proliferation of Human Embro Fibroblast Proliferation and Extracelluar Matrix in Vitro / 中国中医药信息杂志

ABSTRACT

Objective To investigate the effect of puerarin on proliferation of human embro fibroblast and extracelluar matrix. Method Human embro fibroblasts were incubated with 0～400 ?g/mL puerarin for 24～96 h. The MTT method was used to assay the biological activities and extracelluar matrix formation in different time and different dose of pueratin. The cell cycle distribution was detected by flow cytometric analysis. Result Incubation cells in presence of puerarin of 40～400 ?g/mL for 24～72 h could significant inhibit cell proliferation and restrain the collagen synthesis in a dose- and time-dependent manner (P