Construction of EGF coupling bovine serum albumin nano-carrier / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-578277
ABSTRACT
Objective:
To prepare EGF-coupling bovine serum albumin nano-carrier as a new way for initiative targeted drug delivery of epidermal growth factor receptor(EGFR) with broad range of expression in tumor cell.Methods:
albumin nano-carrier was prepared by second phacoemulsification and solvent evaporation technology,and epidermal growth factor(EGF) was conjugated with albumin nano-carrier by chemical crosslinking reagents for EGF coupling bovine serum albumin nano-carrier.Results:
The prepared nano-carrier then underwent isotope labeling,then the coupling effect of EGF and its coupling level were certified by sephadex column and polyacrylamide gel electrophoresis(SDS-PAGE).Conclusion:
Success in the preparation of the smaller bovine serum albumin nano-carrier and EGF coupling bovine serium albumin nano-carrier establish the experimental foundation for further exploration of its targeting of tumor cells in vitro and distribution in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2003
Type:
Article
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