Cloning of human HA117 gene and preparation of itsrecombinant adenovirus vector / 重庆医科大学学报

ABSTRACT

Objective:To construct the recombinant adenovirus expressing human HA117 gene.Methods:The eDNA of HA117 gene was subcloned into the shuttle plasmid pAdTrack-CMV and then the homologous recombinant adenovims genomie plasmid pAdEasy was cloned in bacteria.We tranfected the DNA of identified recombinant plasmid into 293 cells via liposome,made the package and amplification of adenovims,and determined them by fluorescence microscope and flow eytometry.Results:Restriction endonuelease and PCR analysis confirmed that the recombinant adenovims vector of HA117 gene was successfully constructed.The titer of the recombinant adenovims was 8.3?1011PFU/ml,and the expression of HA117 gene was observed.Conclusion:The recombinant adenovims expressing HA117 gene was successfully constructed,which paves the way for the following studies related to HA117.