Preparation and biological characteristics of renal cell carcinoma-dendritic cell fusion vaccine / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-578420
ABSTRACT
Objective:
To prepare renal cell carcinoma-dendritic cell fusion vaccines,and observe the biological characteristics of the fusion cells,to provide theoretical basis and experimental data for further research of DC based cancer vaccines.Methods:
The renal cell carcinoma and dendritic cells were fused by using polyethylene glycol(PEG). The growth curve of fusion cells,morphological characteristics,expression of costimulation molecules,abilities to amplify T lymphocytes and grow into new tumors were detected.Results:
Renal cell carcinoma and dendritic cells could be fused effectively by PEG.The fusion cells had weak proliferation abilities and were unable to grow into new tumors in immunol-deficiency animals,but the costimulation molecules expression such as CD86 and HLA-DR and the abilities to irritate the proliferation of T lymphocytes were remarkably increased than that of parental control groups(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2007
Type:
Article
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