Effects of different concentrations of ?-tocopherol on streptozotocin-induced anti-oxidation competence in pancreatic islet cells / 重庆医科大学学报

ABSTRACT

Objective:To investigate the inhibition effects of different concentrations of ?-tocopherol on pancreatic islet cell viability,insulin secretion,anti-oxidation competence induced by streptozotocin. Methods:Pancreatic islet cells from Wistar rats aged 1 to 3 days were cultured in monolayer in vitro. and divided into 4 groups.Then,cell viability,insulin secretion,T-AOC,SOD activity,MDA level,NOS activity and NO2-/NO3- production were measured by differrent detection methods. Results:Streptozotocin significantly decreased cell viability,T-AOC activity,SOD activity and insulin release level(P0.05),that could not be blocked by ?-tocopherol. Conclusion:?-tocopherol can attenuate the damage of rats pancreatic islet cells induced by streptozotocin,the mechanisms of which may be that ?-tocopherol can reinforce the competence of scavenging free radicals of pancreatic islet cells and not related to NOS activity change.