Observation on protection by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis / 重庆医科大学学报

ABSTRACT

Objective:To investigate protection by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis(Em) and against challenge with Em protoscoleces. Methods:BALB/c mice were vaccinated by mix recombinant BCG vaccines subcutaneously and intranasally respectively,challenged with Em protoscoleces 8w after vaccination and killed 18w after infection to count rate of reduced alveolar echinococcus weight and to examine the level of IgE,IgG and its subclasses. Blank vector,BCG and PBS served as control. Results:In the groups of immunization.The rate of reduced alveolar echinococcus weight was 45.29%～76.47%,the level of IgG,IgG2a,IgG2b and IgE increased apparently,that of IgG1 and IgG3 decreased remarkably. Conclusion:Intranasal vaccination with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine may be a good route,IgG,IgG2a,IgG2b and IgE may play an important role in protection induced by this vaccine.