Expression and role of AQP9 in models of cultured steatosis hepatocytes / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-578566
ABSTRACT
Objective:
To explore the expression and role of AQP9 in the models of cultured steatosis hepatocytes.Methods:
Steatosis models of hepatocytes was established by adding oleic acid to the growing L-02 cell strain.The expression of AQP9 mRNA was measured with RT–PCR and the protein expression of AQP9 were measured by Western blotting.Lipid droplets in the hepatocytes were observed by oil red staining and the contents of triglyceride,FFA and glycerol in hepatocytes were measured with analyzed kit.Results:
Through oil red staining.a few lipid droplets were observed at 24h and steatosis hepatocyte increased greatly at 72h.Triglyceride,FFA and glycerol contents in hepatocytes of model groups also increased,as compared with the control group(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2007
Type:
Article
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