Effects of Jiejufushenning on advanced glycation end products in serum and expression of receptor for advanced glycation end products in kidney in diabetic rats / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effects of Jiejufushenning on advanced glycation end products(AGEs)in serum and expression of receptor for advanced glycation end products(RAGE) in kidney in diabetic rats.Methods:Diabetic SD rats were induced by injection of streptozotocin,then the diabetic rats were randomly divided into four groupsdiabetic control group(group B),Jiejufushenning group(group C),aminoguanidine group(group D),Jiejufushenning and aminoguanidine group(group E),age-matched norma1 SD rats served as norma1 contro1 group(group A).After 12 weeks of treatment by corresponding intervention,blood glucose,renal function,the quantitation of urine protein of 24 hours(24hPro)and urine creatinine were determined by the normal methods.The levels of AGEs in serum were determined by fluorospectrophotometry,and the expressions of RAGE in kidney were determined by immunohistochemical methods.Results:The levels of blood glucose,urea nitrogen(BUN),serum creatinine(Scr)and 24hPro in rats of group C,group D and group E were obviously lower than those of group B,but higher than those of group A;The levels of urine creatinine of group C,D and E were obviously higher than that of group B(P