The effect of hnRNP K shRNA on K562 cells / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-578670
ABSTRACT
Objective:
To explore the effect of heterogeneous nuclear ribonucleoprotein K(hnRNP K)short hairpin RNA(shRNA)on K562 cells.Methods:
hnRNP K shRNA retrovirus was packaged with pSUPER retro hnRNP K shRNA after its identification by enzyme digestion and sequencing analysis,then FCM,Wright staining,MTT assay,RT-PCR were used to analyze its effects on cell cycle and apoptosis,proliferation,hnRNP K and eukaryotic translation initiation factor-4E(eIF4E) mRNA of K562 cells respectively.Results:
The sequence of the inserted fragment in pSUPER retro hnRNP K shRNA was correct. Compared with the control K562 cells,hnRNP K shRNA treated group showed significantly more cells at G2/M phase(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2007
Type:
Article
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