Rapid determination of lumbrukinase and its hydrolysates in processed earthworm by dot blotting / 中草药

ABSTRACT

Objective To determine the lumbrukinase contents and its hydrolysates in processed earthworm,set up a new evaluating method of dot blotting to evaluate the qualities of those processed Chinese medicines as earthworm. Methods Contents of lumbrukinase and its hydrolysates in processed earthworm were determined by dot blotting with rabbit antibody of anti-lumbrukinase as the first antibody. Blotting signal was analyzed by Quantity One software. Results Contents of lumbrukinase and its hydrolysates in processed earthworm of Pheretima aspergilum,P. pingi,Drawida japonica,and D. gisti were 125.23,118.34,81.05,and 72.13 ?g/g,respectively. Conclusion There was no significant difference between the processed earthworms of P. aspergilum and P. pingi in the content of lumbrukinase and its hydrolysates,both of which were higher than those of the other two species in Shandong. Theoretically,the processed products of P. pingi could be used as the succedaneum of P. aspergilum in the clinical of traditional Chinese medicine. Dot blotting,as a convenient and accurate method,could be broadly used for the evaluation of the processed products of Chinese crude drugs similar to earthworm.