Effect of Tongmai Oral Liquid on Renal Pathological Feature and Tumor Growth Factor ?_1 in Rats with Experimental Diabetic Nephropathy / 广州中医药大学学报

ABSTRACT

Objective To observe the effect of Tongmai Oral Liquid(TOL) on renal pathological feature and tumor growth factor ?1(TGF-?1) in rats with experimental diabetic nephropathy(DN).Methods Forty SD male rats were used for the experiment.Of which,8 were in the normal group,and the other rats were given one-dose intraperitoneal injection of streptozocin(STZ),and were fed with high-fat forage every other day.The normal group received one-dose intraperitoneal injection of aseptic citric acid-sodium citrate buffer and routine forage.After feeding for 4 weeks,the rat models of DN were randomized into the model group and TOL group,which were given normal saline and TOL(2.5 g?kg-1?d-1) respectively,and the two groups were still fed with high-fat forage.After treatment for 6 weeks,the rats were executed and the pathological features and ultrastructure of renal tissue were observed under light microscope and electron microscope respectively.Mean glomerular area(MGA),mean extracellular matrix area(MMA),and the thickness of glomerular basement membrane(GBM) as well as the ratio of MMA/MGA were measured.Meanwhile,the expression of tumor growth factor ?1(TGF-?1) was detected by immunohistochemical method.Results In TOL group,the pathological features such as broadened mesentery,thick basement membrane and vacuolar degeneration in the renal tubular epithelium were relieved,MMA/MGA ratio was decreased,GBM became thin,and the expression of TGF-?1 was strong mainly in renal tubular and interstitial region while weak in glomeruli,and its area density and integral optimal density were decreased as compared with the model group(P