Effect of human cytokine-induced killer(CIK)cells on the model of human hepatocellular carcinoma in nude mice(LCI-D20) / 重庆医科大学学报

ABSTRACT

Objective:To investigate the cure effect of human cytokine-induced killer (CIK) cells on the model of human hepatocellular carcinoma in nude mice. Methods:Peripheral blood mononuclear cells(PBMC)were obtained from healthy volun- teers by Ficoll gradient centrifugation and CIK cells were induced by culturing PBMC in the completed medium containing rhIFN-?,rhIL-2 and mAb against CD3.By intraperitoneal injection of CIK cells in LCI-D20, the effect of prevention and therapy to HCC in LCI-D20 were assessed. Results:The weight of tumor in the CIK cells group and negative group was (1.125?0.363)gram and(2.682?0.526)gram respectively(t=6.06,P