Comparative pharmacokinetics of active component in Guanxin Suhe Pills containing Radix Inulae in place of Radix Aristolochiae / 中草药

ABSTRACT

Objective To study the pharmacokinetics of the active component in series of Guanxin Suhe Pills including the prescription containing Radix Aristolochiae(QG),the prescription containing Radix Inulae(TG),and the prescription without any of them(BG) in Beagle dogs and to compare the pharmacokinetics among the series of prescription.Methods HPLC Method was developed to determine acid in plasma of Beagle dogs.Blood plasma was collected after series of Guanxin Suhe Pills were ig administrated to Beagle dogs 0—8 h later.The concentration of cinnamic acid in the plasma was determined and DAS 2.1 software was used in calculation of compartment model and pharmacokinetic parameters.Results The standard curve was linear from 0.031 25 to 32 mg/L in plasma of Beagle dogs,the lowest detectable limit was 31.25 ?g/L,the recovery rate of the method was over 90 % with satisfactory relative standard deviations(RSDs) of intra-day and inter-day both lower than 5 %.The metabolism of cinnamic acid in plasma of Beagle dogs after medication of series in QG,TG,and BG all fitted in a first order absorption of two-compartment model.The peak concentration and the area under curve in TG and BG groups had the higher tendency than those in the QG group,but there was no significant difference among the three groups.Conclusion The series of Guanxin Suhe Pills all have a similar pharmacokinetic process.Taking away the Radix Aristolochiae from the previous prescription that including the Radix Aristolochiae or taking the place of the Radix Aristolochiae with Radix Inulae does not influence the pharmacokinetic process of the main active component.